Kjeldahl Method (The Gold Standard of determination of Nitrogen Content)
Overview of the Kjeldahl Method
The Kjeldahl method is a standard laboratory technique used to determine the nitrogen content in various substances, including food, feed, fertilizers, and soil. It is widely applied due to its reliability and precision, especially in estimating protein content based on nitrogen content.
Principle
The method works by converting organic nitrogen in a sample into ammonium ( 𝑁 𝐻 4 + NH 4 + ) during digestion with concentrated sulfuric acid. The nitrogen is then quantified by measuring the amount of ammonia ( 𝑁 𝐻 3 NH 3 ) released after neutralization and distillation.
Kjeldahl Method (The Gold Standard of determination of Nitrogen Content)
Overview of the Kjeldahl Method
The Kjeldahl method is a standard laboratory technique used to determine the nitrogen content in various substances, including food, feed, fertilizers, and soil. It is widely applied due to its reliability and precision, especially in estimating protein content based on nitrogen content.
Principle
The method works by converting organic nitrogen in a sample into ammonium ( 𝑁 𝐻 4 + NH 4 + ) during digestion with concentrated sulfuric acid. The nitrogen is then quantified by measuring the amount of ammonia ( 𝑁 𝐻 3 NH 3 ) released after neutralization and distillation.